Cloning and Expression of Helicobacter pylori ulcer Associated Gene - iceA1 in Brinjal (Solanum melongena L.)

Background: Plant biotechnology revolutionized the branch of plant sciences contributing to improving crop production. Helicobacter pylori was predominantly seen among all sectors human population, closely stuck stomach ulcers and cancer. Even though traditional vaccines are effective in preventing H. pylori, edible non-edible parts special transgenic plants were also being generated express its antigens which could elicit an immune response after consuming. The induce by contact with epithelium (iceA1) protein is associated virulence stated as predisposing factor Objectives: This study intends expression levels iceA1 gene brinjal plant. Materials Methods: present study, magnified from strain 26695 transformed it into callus (from leaf explants) through Agrobacterium tumefacians (EHA105). pBI121 vector used construct vector, further validated amplification studies. Results: Out 46 obtained this five them found be positive for on validation assays like Conclusion: beneficially produce a vaccine candidate against brinjal. In Addition, provide, valid scientific data can researchers.